Restricting User ‘malawi’ from Installing System-wide Programs and Verifying Permissions

 chmod o-rx /home/jhuang
 ls -ld /home/jhuang
 cd /home/jhuang

 groups malawi
 sudo deluser malawi sudo

 jhuang@hamm:~$ groups malawi
 #malawi : malawi
 jhuang@hamm:~$ groups jhuang
 #jhuang : jhuang adm cdrom sudo dip plugdev lpadmin sambashare docker

Ensuring Full Permissions for User ‘malawi’ in Their Home Directory

 #chmod -R u=rwx,go= /home/malawi
 ls -ld /home/malawi
 #drwxr-xr-x
 sudo chown malawi:malawi /home/malawi
 #sudo chmod 700 /home/malawi
 #sudo chmod -R 700 /home/malawi
 sudo chmod 755 /home/malawi
 sudo chmod -R 755 /home/malawi
 ls -ld /home/malawi

Protecting Other Users’ Directories from Access by ‘malawi’

 sudo chmod o-rx /home/jhuang
 sudo chmod o-rx /mnt/h1/jhuang

 #for dir in /home/*; do
 #  if [ -d "$dir" ]; then
 #    sudo chmod o-rx "$dir"
 #  fi
 #done

 ls -ld /home/jhuang

Changing User Passwords

 sudo passwd malawi
 passwd

Changing User Passwords

 if (!requireNamespace("BiocManager", quietly = TRUE))
     install.packages("BiocManager")
 BiocManager::install("SeuratObject")
 install.packages("Seurat")

 #(NOT GOOD) from 775 to o-rx 
 #sudo chmod o-rx /mnt/h1/jhuang
 #sudo chmod -R o-rx /mnt/h1/jhuang
 #sudo chmod o-rx /home/jhuang
 #sudo chmod -R o-rx /home/jhuang

 #from 775 to 750
 sudo chmod 750 /mnt/h1/jhuang
 sudo chmod -R 750 /mnt/h1/jhuang
 sudo chmod 750 /home/jhuang
 sudo chmod -R 750 /home/jhuang
 #END

install r and r-seurat with miniconda3

 mkdir -p ~/miniconda3
 wget https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh -O ~/miniconda3/miniconda.sh
 bash ~/miniconda3/miniconda.sh -b -u -p ~/miniconda3
 rm -rf ~/miniconda3/miniconda.sh
 ~/miniconda3/bin/conda init bash

 conda create -n r -c bioconda r r-seurat
 conda activate r
 #> .libPaths()
 #[1] "/home/malawi/miniconda3/envs/r/lib/R/library"
 if (!requireNamespace("remotes", quietly = TRUE))
   install.packages("remotes")
 remotes::install_github("satijalab/seurat-data")
 conda deactivate

 conda activate r
 R
 library(Seurat)
 library(SeuratData)
 library(ggplot2)
 library(patchwork)
 library(dplyr)