Restricting User ‘malawi’ from Installing System-wide Programs and Verifying Permissions

chmod o-rx /home/jhuang
ls -ld /home/jhuang
cd /home/jhuang

groups malawi
sudo deluser malawi sudo

jhuang@hamm:~$ groups malawi
#malawi : malawi
jhuang@hamm:~$ groups jhuang
#jhuang : jhuang adm cdrom sudo dip plugdev lpadmin sambashare docker

Ensuring Full Permissions for User ‘malawi’ in Their Home Directory

#chmod -R u=rwx,go= /home/malawi
ls -ld /home/malawi
#drwxr-xr-x
sudo chown malawi:malawi /home/malawi
#sudo chmod 700 /home/malawi
#sudo chmod -R 700 /home/malawi
sudo chmod 755 /home/malawi
sudo chmod -R 755 /home/malawi
ls -ld /home/malawi

Protecting Other Users’ Directories from Access by ‘malawi’

sudo chmod o-rx /home/jhuang
sudo chmod o-rx /mnt/h1/jhuang

#for dir in /home/*; do
#  if [ -d "$dir" ]; then
#    sudo chmod o-rx "$dir"
#  fi
#done

ls -ld /home/jhuang

Changing User Passwords

sudo passwd malawi
passwd

Changing User Passwords

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")
BiocManager::install("SeuratObject")
install.packages("Seurat")

#(NOT GOOD) from 775 to o-rx 
#sudo chmod o-rx /mnt/h1/jhuang
#sudo chmod -R o-rx /mnt/h1/jhuang
#sudo chmod o-rx /home/jhuang
#sudo chmod -R o-rx /home/jhuang

#from 775 to 750
sudo chmod 750 /mnt/h1/jhuang
sudo chmod -R 750 /mnt/h1/jhuang
sudo chmod 750 /home/jhuang
sudo chmod -R 750 /home/jhuang
#END

install r and r-seurat with miniconda3

mkdir -p ~/miniconda3
wget https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh -O ~/miniconda3/miniconda.sh
bash ~/miniconda3/miniconda.sh -b -u -p ~/miniconda3
rm -rf ~/miniconda3/miniconda.sh
~/miniconda3/bin/conda init bash

conda create -n r -c bioconda r r-seurat
conda activate r
#> .libPaths()
#[1] "/home/malawi/miniconda3/envs/r/lib/R/library"
if (!requireNamespace("remotes", quietly = TRUE))
  install.packages("remotes")
remotes::install_github("satijalab/seurat-data")
conda deactivate

conda activate r
R
library(Seurat)
library(SeuratData)
library(ggplot2)
library(patchwork)
library(dplyr)