Logics of Transposon sequencing

original reads:
>read_1/1
    AAGAGTTAGTAGGGCGGGCGATTAAGGGCAAACGTGATCAGGTCTTTTTAGCGACTAAGT
TCGGTATTGTGCGTGACCCCACTGATCCCAGGGTACGCGGTGTGAGCAGCCGTCCTGATT
ATATCCGCCAGTCG  GTTGATGAGAGCTTTGTTGTAGGTG

TODO_TOMORROW: follow the logics below!
vs. NEB_i5_Tn_02
5'-ACACTCTTTCCCTACACGACGCTCTTCCGATCTCACCTACAACAAAGCTCTCATCAAC-3'-bacterial_genomics
duplicate_PCR generate rc fragments
                 adapter_removed!  |GTGGA  ...     AGAGTAGTTG                       ...  GATTGAGAA
                                                                                 <-------read_R1--

>read_1/2
AATGAAGAGTTAGTAGGGCGGGCGATTAAGGGCAAACGTGATCAGGTCTTTTTAGCGACT
AAGTTCGGTATTGTGCGTGACCCCACTGATCCCAGGGTACGCGGTGTGAGCAGCCGTCCT
GATTATATCCGCCAGTCGGTTGATG  AGAGCTTTGTTGT

like unlike

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