AmpliconNoise is a collection of programs for the removal of noise from 454 sequenced PCR amplicons. Typical usage involves three steps:
1)the removal of noise from the sequencing itself,
2)the removal of PCR point errors, and
3)removal of chimeric sequences using the program Perseus.
While steps 2-‐3 can be used on amplicon reads generated using most sequencing technologies, step 1 is currently only supported for sequences generated using 454 pyrosequencing.
When presenting work using AmpliconNoise, please refer to the following citation:
Quinceet al (2011), ‘Removing noise from pyrosequenced amplicons.’, BMC Bioinformatics12, 38